Photothermal therapy (PTT) is one of the most efficient therapies that can induce necrosis of specific malignant lesions with minimal invasiveness and side effects compared with other therapeutic modalities.
In this context, applications are open for a postdoctoral fellowship position funded by the “FireLight” projet-FEDER – Photoactive molecules and nanoparticles.
The position will be based at L2CM (http://www.l2cm.univ-lorraine.fr/l2cm/), CRAN (http://www.cran.univ-lorraine.fr/) and ICL (http://www.icl-lorraine.fr/) laboratories in Nancy (France).
The research program will focus on the development of indocyanine green (ICG) loaded Solid Lipid Nanoparticles (SLN) and on the characterization of photophysical (size and charges measurements, spectral characterization, thermal effect) and photobiological properties (uptake, intracellular fluorescence and localization, therapeutic efficiency). The biological model used will be 2D and 3D cell cultures and animal models.
Selection process: Interested candidates should send a CV, a scientific track record, a motivation letter and two recommendation letters to:
Dr. Henri-Pierre Lassalle / Prof. Andreea Pasc
Contact: firstname.lastname@example.org; email@example.com
Skills/Qualifications: Candidates for this postdoctoral fellowship must hold a PhD degree in cell biology, good knowledge in photobiology will be required, as well as experience in animal experiments (the authorization to experiment on animals will be highly appreciated). Creativity, autonomy and strong reliability are highly required. All applicants must be able to communicate fluently in French and/or English (speaking and writing). The position is available for a period of 18 months, starting from September 2020.
Keywords: Cancer, Nanoparticle, Photothermal therapy (PTT), NIR fluorescence, Indocyanine green, Photodiagnosis.
CRAN - Centre de Recherche en Automatique de Nancy
ICL - Institut de Cancérologie de Lorraine
Université de Lorraine
Background: This request is made in the context of the development of photoactive molecular systems for health, in particular for applications in phototherapy, opening the way to the treatment of many pathologies, including those of infectious and cancerous origin, using light.
Recent work carried out in the laboratory concerning the preparation of potential macro-bis-heterocyclic bis-imidazolium N-heterocyclic carbene (NHC) ligands, have shown interesting intrinsic photophysical and biological properties of these molecules, with a production of singlet oxygen (1O2), in yields of up to 84% for some of the macrocycles obtained. We wish to continue these syntheses in order to modify the structures and improve their photoactive properties with a shift towards higher wavelengths (> 800 nm) in particular for applications in phototherapy. We aim at preparing structures with a higher conjugation but also a larger size allowing a better coordination of transition metals (e.g. Zn (II), Ag (I), Au (III), Pt (II), Fe (II)).
Objectives: The Master 2 subject will concern the organic synthesis of original N-macro-bis-heterocyclic carbene ligands and the metal cation complexes of the isolated compounds (scheme above), as well as their characterization.
Methodology: The candidate will perform organic synthesis reactions and characterization of the isolated molecules by classical physicochemical methods (Infra Red (IR), Nuclear Magnetic Resonance (NMR), mass spectroscopy, microanalysis, etc.). The study of their coordination properties will then be carried out. Depending on the progress of the synthesis, the analysis of the photo-physical properties (fluorescence, 1O2, photoacoustics) and the application in biology (cytotoxicity, antimicrobial/antiviral properties, antiproliferative) will then be discussed in collaboration with Dr. Mihayl VARBANOV.
Desired profile: The candidate should have a strong knowledge in organic synthesis methodology and coordination chemistry. He/she should have the necessary knowledge of physico-chemical analysis (IR, NMR, mass spectroscopy, microanalysis). Good knowledge in biology is welcome. For international candidates, a good command of English is sufficient (a good knowledge of French would be appreciated).
Application: Applications should be sent to Florence Dumarçay (firstname.lastname@example.org) and must include a CV and the transcript of records of BSc and MSc levels.
Background: The development of photoactive molecular systems, with application in phototherapy, opens the way to the treatment of many pathologies, including those of infectious and cancerous origin, using light. Recent work carried out in the laboratory concerning the preparation of potential macro-bisheterocycles bis-imidazolium ligands NHC (N-heterocyclic carbene) in coordination with transition metals (e.g. Zn (II), Ag (I), Au (III), Pt (II), Fe (II)), has shown interesting intrinsic photophysical and biological properties of these molecules. These observations concern particularly the production of singlet oxygen (1O2), the yield of which can reach 84% for some of the macrocycles obtained, as well as the antibacterial activities. We wish to continue the biological characterization of these structures in order to improve their photoactive properties, in particular for applications in phototherapy (PT).
Objectives: The objective of the traineeship will concern the biological characterization of the original N-macro-bis-heterocyclic carbene ligands and the metal cation complexes of isolated compounds (illustrated above). The focus will be on evaluating the anti-infectious properties of the molecules, in presence or absence of irradiation.
Methodology: The candidate will carry out the evaluation of the antibacterial (pathogenic strains, microbiota strains) and antiviral (coronavirus, herpesvirus) properties of the compounds, as well as the evaluation of their impact on the host cells (cytotoxicity, hematotoxicity). The link with photo-physical (fluorescence, 1O2, photoacoustic), physico-chemical and structure-activity relationships will then be discussed in collaboration with Dr. Florence Dumarçay.
Profile of the candidate: The candidate should have solid knowledge in bacteriology / virology and cell culture. He must have the necessary knowledge for analyzes in cell biology (light microscopy, immunofluorescence, FACS, Western blot), molecular biology (PCR, RT-PCR) and biochemistry. Good knowledge in chemistry / physical-chemistry will be welcome. For international applicants, fluency in English is sufficient (a good foundation in French will be appreciated).
Application: Applications should be sent to Mihayl Varbanov (email@example.com) and must include a CV and the transcript of records of BSc and MSc levels.